DCO Project Summary

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Project Title
Characterization of a Novel, Thermophilic, Subsurface Bacterial Genus Related to Candidatus Desulforudis audaxviato
Start DateEnd Date
2014-04-01 2014-12-31
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This project will characterize the first cultivated representative of a novel bacterial genus (denoted DRI-14) isolated from a deep, regional aquifer beneath the Nevada National Security Site (NNSS). This thermophilic anaerobe may have particular relevance to all three of the decadal goals of the Deep Life Community (DLC) with a particular focus on Goal 3: “Determine the interaction between deep life and carbon cycling” due to DRI-14’s apparent physiological linkage to the major source of mobile carbon in the deep biosphere (e.g., it grows in pure culture with methane as the sole C and energy source) and on Goal 1: “Determine the processes that define the diversity and distribution of deep life as it relates to the carbon cycle” as its status as the nearest cultivated neighbor (91% over the full-length 16S rRNA gene) to Candidatus Desulforudis audaxviator.
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Reporting Year 2014 Click to expand

  • RY2014-1 - submitted on Aug 31, 2014

    Update Details:

    Progress: Characterization work on strain DRI-14 commenced on April 1, 2014. To date, the work has focused on: (a) optimization of growth media formulation, (b) determination of optimal growth parameters (temperature, pH, energy substrates), (c) phylogenetic analysis/genomic DNA sequencing, and (d) the tracking of carbon incorporation using 13C-labeled methane.

    Conclusion/Discussion: With improvements to the medium preparation and composition, we are ready for another stable isotope probing study to characterize the utilization of CH4 anaerobically by strain DRI-14. If this experiment is successful, we plan to submit to a high-level journal such as Science or Nature. Characterization and genome information will be submitted in a manuscript to Environmental Microbiology or PLOS ONE (Note: name of organism has not been finalized).


    Publications in preparation:
    Hamilton-Brehm S. D., Orphan V., and Moser D. (~2014) “Anaerobic Oxidization of methane conducted by an isolated bacterium from the Nevada National Security Site” [Proposed submission to Science or Nature?]
    Hamilton-Brehm S. D., Stewart L., Vineis J. H., Orphan V., and Moser D. (~2015) “Desulfogeovirga methanotrophicus DRI-14T sp. a novel anaerobic methane oxidizing thermophilic bacterium isolated from 800 meters underground the Southern Nevada Desert” [Proposed submission to Environmental Microbiology? PLOS ONE?]

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