DCO Project Summary

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Project Title
Single-Cell Genomics of Deep Fracture Microbial Communities
Start DateEnd Date
2012-01-01 2014-06-30
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The Deep Fracture (DF) portion of the Rock Hosted Communities Project sought to build upon previous studies on the microbial diversity of Precambrian Shield deep ecosystems and use cell sorting and single-cell genome sequencing approaches to evaluate the adaptations and the population genetics of these deep isolated communities. The work built primarily upon sampling opportunities in South Africa and Finland where populations of the deep biosphere organism Candidatus Desulforudis audaxviator were found to be prevalent.

Project UpdatesClick to add Project Update

Reporting Year 2014 Click to expand

  • RY2014-1 - submitted on Aug 31, 2014

    Update Details:

    Magnabosco, C., C.Y.M. Lau, T.C. Onstott, T.L. Kieft, M. Tekere, J. Olivier, B. Linage, O. Kuloyo, M. Erasmus, E. Cason, E. van Heerden, and G. Borgonie, Comparisons of the composition and biogeographic distribution of the bacterial communities occupying South African thermal springs with those inhabiting deep subsurface fracture water. Frontiers in Microbiology, submitted.

    Labonté J, E.K. Field, M. Lau, E. Van Heerden, T.L. Kieft T.C. Onstott, R. Stepanauskas R. Genetic variability of Candidatus Desulforudis audaxviator in the deep subsurface. In preparation. 

    Lau, M.C.Y., C. Cameron, C. Magnabosco, C.T. Brown, F., Schilkey, S. Grim, S. Hendrickson, M. Pullin, G. Lacrampe-Couloume, B. Sherwood Lollar, Esta van Heerden, T.L. Kieft and T.C. Onstott, Phylogeny and phylogeography of functional genes shared among seven terrestrial subsurface metagenomes reveal N-cycling and microbial evolutionary relationships. Frontiers in Microbiology, submitted.

  • RY2014-2 - submitted on Aug 31, 2014

    Update Details:

    Cells were sorted from samples obtained from the South African deep fracture ecosystem as well as metabolically “activated” cells from the Outokumpo borehole in Finland. Populations of D. audaxviator and relatives were identified and their sequences have been obtained and are currently in the process of bioinformatics analyses. In parallel, samples for the enrichment of piezophilic isolates were obtained.

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http://info.deepcarbon.net/individual/n2911 11121/3731-3787-1604-7520-CC
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