DCO Project Summary

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Project Title
IODP Leg 347: Baltic Sea Paleoenvironment
Start DateEnd Date
2013-09-12 2013-11-01
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As part of the onshore science party in IODP Expedition 347: Baltic Sea Paleoenvironment, we are sequencing single cell genomes of uncultured archaea and bacteria in sediments as deep as 85 m. The vast majority of the microbial diversity in deep subsurface environments has eluded efforts to be cultured. Single cell genomics affords us the opportunity to uncover new prospective ecophysiological roles carried out by enigmatic yet abundant organisms. We now have 40 single amplified genomes (SAGs) from 37.5 mbsf and 20 SAGs from 85 mbsf at Baltic Sea Basin hole 60B. The SAGs include members of the deeply-branching archaeal and bacterial groups lacking cultured representatives. To our knowledge, these are the first SAGs ever successfully obtained from deep (> 10 mbsf) marine sediments. We currently have funding to sequence 10 SAGs. Adding 3 more would therefore greatly expand our understanding of the genetic composition of uncultured microorganisms in deep Baltic Sea sediments.
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Reporting Year 2015 Click to expand

  • Update 2015: IODP Leg 347: Baltic Sea Paleoenvironment - submitted on ,

    Update Details:

    Update Submitted by Karen Lloyd, October 2015

    We obtained sequences from three single cells whose 16S rRNA genes designate them as uncultivated members of the Chloroflexi phylum. These single cells were sorted at the Single Cell Genomics Center and, since they were derived from 85 meters below the seafloor, they may be the deepest single cell genomes from Chloroflexi to date. All three genomes appear free from contamination of a second genome, giving us high confidence that, although the genomes are incomplete, they each represent pieces of single genomes. Work is on-going to place them in an environmental and biogeochemical context.

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